Emphaze™ AEX Hybrid Purifer can optimize the purification process of mammalia$n cell culture products and downstream monoclonal antibodies (mAbs) by protecting Protein A affini×ty chromatography column and prolonging its service life.
Characteristics of Emphaze™ AEX Hybrid Purifer:
1. Improve process efficiency
lRemove multiple types of impurities through a one-stΩep process, thereby greatly streamlining the customer’s process αand achieving better process benefits.
Zeta Plus™ Series Depth Filter, Emphaze™ AEX Hybrid Purifer and LifeASSUΩRE™ Membrane as matching filtration solutions can≠ replace centrifugal unit operation and reduce the used area of do♥wnstream sterilizing membrane.
2. Improve product purity and yield
The matching filtration solution provided by 3M can remove impurity partic↔les early in the process, protect the chromatography column and improve its purificat&ion performance.
3. Simplify the production process
Emphaze™ AEX Hybrid Purifer is a fully synthe↑tic, high loading purification product consisting of Q-func↑tional nonwoven media and bioburden reducing membrane.
Only buffer or salt solution is recommended for Emphaze™ AEX Hybrid Purifer pr§econditioning flush, not deionized water.
Process Advantage of Emphaze™ AEX Hybrid Purifer
Turbidity reduction
Based on the Emphaze™ AEX Hybrid Purifer products have strong anion exchange abil≠ity and mixing mechanism of 0.2μm membrane at the terminal, so its turbid♥ity reduction ability is better than that of the conventional; depth filtratio←n. During the whole purification process, the turbidity of the filtrate is generally <5NTU.
Figure 1: Pressure-Load and Turbidity-Load curves during the purification process o×f CHO centrifugate
HCP reduction
The positive charge of the Q-functional nonwoven mediaα bonded to Emphaze™ AEX Hybrid Purifer can significantly reduce the negatively charged HCP. The HCP removal rate of harvest and centrifugate can usually reach 20-4§0%.
Figure 3: Pressure-Load and HCP removal rate-Load curves duri≤ng the purification process of CHO centrifugate
DNA reduction
Compared with conventional depth filtration, Emphaze™ AEX Hybrid Purifer has mu$ch higher DNA capture ability. Usually, the DNA LVR＞4 in harvest and centrifugate supernatant.
Figure 2: Pressure-Load and DNA removal rate-Load curves during the purification process of CHO c↑entrifugate
Bioburden reduction
The unique design of integrate the Emphaze™ AEX Hybrid Purifer with 0.2μm membran®e can provide bioburden LRV＞6, and provide excellent protection for downstream ste≠rilizing membranes (including 0.1μm sterilizing membranγes for reducing mycoplasma). This is also an economical strategy to reduce the risk of downsπtream pollution caused by bacteria and mycoplasma.
Table 1: Challenge test results of defective pseudomonas
Challenge condition 1: Mother liquor of challenger bac∏teria diluted with physiological saline, 2L (ASTM F838-05)
Challenge condition 2: Undiluted challenge bacteria ✘mother liquor, 6L.
Excellent throughput and mAb recovery
Emphaze™ AEX Hybrid Purifer can not only substantially reduce turbidity, ₹DNA, HCP and bioburden, but also have the same throughput as conventional high-precision depth fil¶ter (see Figure 1-3). In addition, as an all-synthetic, low nonspec×ific binding and positively charged Q-functional nonwoven €media purification product, it can provide better mAbs yield than depth filtration.
Improving the performance of capture column
Emphaze™ AEX Hybrid Purifer can effectively reduce pre Prote₹in A Column soluble impurities, such as DNA and HCP, thus greatly improving the performance of the> capture column. That is, after using Emphaze™ AEX' Hybrid Purifer in the upstream purification process,λ the capture column can remove HCP and DNA more effectively.
Enhance the protection to capture column
Emphaze™ AEX Hybrid Purifer has excellent protection ability against expensive capture chromatography, and greatly reduces the impurΩities bound to chromatography column. So, a milder column cleaning c"ondition can be adopted, which can greatly prolong the se₽rvice life of the chromatography column.
Application of Cell Harvest & Clarification
Emphaze™ AEX Hybrid Purifer, combined with Z≈eta Plus™ Series Depth Filter and LifeASSURE&tradeβ; membrane as a matching filtration solution, can greatly improve the process≤ and make it possible to operate without centrifugation.
• HCP remove rate is 30%, DNA LRV >4
• Consistent output turbidity <5 NTU
• Output particle size distribution <30nm
• Bioburden LRV >6
Application of Capture, Fine Purification, and Final Filling÷
The performance of Emphaze™ AEX Hybrid Purifεer can reduce the use scale of fine chromatography columns for s÷ubsequent removal of soluble impurities such as DNA, HCP a♥nd viruses. 3M Emphaze™ AEX Hybrid Purifer gr€eatly improved the whole production efficiency by sλignificantly reducing the soluble impurities such as DNA &and HCP in the samples on the capture column.
Emphaze™ AEX Product Selection/Specification
Guochu Technology (Xiamen) provides products of 3M, including: Harvest RC Series Filter, ¥Emphaze™ AEX Series Chromatography Filter, Polisher ST Series Chromatography Filt←er, LifeASSURE™ PDA Filter Cartridge, LifeASSURE™ 'PFS Filter Cartridge, LifeASSURE™ BDA Filter Cartridge, Zeta Plus&tra★de; Filter Cartridge, LifeASSURE™ BA Series Filter Cartridge, Lif♣eASSURE™ BNA Filter Cartridge, BetafineT™ DP Filter Ca rtridge, BetafineT™ XL Filter Cartridge, BetafineT™ BT Filter Cartridge, Micro-δKlean™ RT Filter Cartridge, Micro-Klean™ D Filter Cartridg₽e, Betapure™ AU Filter Capsule, Betapure™ N×T-T Filter Capsule, 3M™ High Flow Filter Cartridge, 3M&₹trade; NB Filter Bag, Micro Screen™ Metal Filter Cartridge, Liqui-Cel™ Membrane Contactor, Liqui Flux™ B Cross-flow Membrane, ♠3M™ CFSI Activated Carbon Filter Cartridge, ZW Series Cartr<idge, Zeta Plus™ Series Cartridge, Sanitary High Flo™w Cartridge, etc.
For details, please contact +86-592-6518670, +86-592-6514970.
Guochu Technology (Xiamen) provides all models of 3M Emphaze™AEX Hybrid Purifer Series,β including: EMP301AEX020A, EMP303AEX020A, EMP513AEX020A, EMP7∞10AEX020A, EMP770AEX020A.
